History of assay achievements
A major research focus within the Radboud University Medical Centre is on immune-regulation and immunotherapy.
For monitoring of KLH-containing cancer vaccines we recently developed the first quantitative-assay to detect human anti-KLH antibodies in serum/plasma (Aarntzen et al. CII 2012).
In recently published articles we describe our ELISA method and diverse applications of anti-KLH analysis in clinical trials:
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Title article |
Reference |
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Simultaneous presence of non- and highly mutated KLH-specific plasmablasts early after primary KLH immunization Monitoring of dynamica changes in KLH-specific B cells in KLH-vaccinated cancer patients Intranodal vaccinations with mRNA-optimized dendritic cells in metastatic melanoms patients Targeting CD4+ T helper cells improves the induction of anti-tumor responses in dendrtitic cell based vaccination |
Giesecke et al. JI 2018 Wimmers et al. Sci Rep 2017 Bol et al. Oncoimm. 2015 |
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Skin-test infiltrating lymphocytes early predict clinical outcome of dendritic cell-baed vaccination in metastatic melanoma |
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Vaccination with mRNA-eclectroporated dendritic cells induces robust tumor antigen-specific CD4+ and CD8+ T cells responses in stage III and IV melanoma patients |
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Humoral anti-KLH responses in cancer patients treated with dendritic cell-based immunotherapy are dictated by different vaccine parameters. |
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Early identification of antigen-specific immune responses in vivo by [18F]-labeled 3'-fluoro-3'-deoxythymedine PET imaging |
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Route of administation modulates the induction of dendritic cell vaccine-induced antigen-specific T cells in advanced melanoma patients |
